This ebook reveals the provision and potential of the coupled acoustic-gravitational (CAG) subject for trace-level biosensing. The proposed detection scheme additionally permits the analysis of the kinetics and thermodynamics of the response occurring on a single microparticle (MP). This methodology has broad applicability in necessary fields, involving not solely chemistry but in addition life, environmental, and medical sciences.
The writer proposes novel trace-level biosensing based mostly on measurements of the levitation coordinate shift of an MP within the CAG subject. The levitation coordinate of the MP within the CAG subject is set by its density and compressibility. The levitation coordinate shift is induced by the binding of gold nanoparticles (AuNPs) to the MP by way of interparticle reactions. Due to this fact, the amount of molecules concerned within the response may be decided from the levitation coordinate shift. The writer demonstrates the zmol degree detection for biotin, DNA/RNA, and natural molecules. As well as, the kinetics and thermodynamics are evaluated for varied reactions occurring between the MP and AuNP, such because the avidin-biotin response, direct hybridization, sandwich hybridization, and aptamer-target complexation.
This ebook gives a brand new idea based mostly on the CAG subject, during which the extent of a response is transformed into the levitation coordinate shift, that’s, “size.” The proposed methodology has many benefits over different strategies, e.g., excessive biocompatibility, excessive applicability, and brief evaluation time. As well as, as a result of the equipment used on this research is cheap and simple to miniaturize, this methodology is helpful in necessary sensible fields, akin to forensic and environmental science and analysis. Thus, this ebook evokes many researchers to use the current methodology to their very own fields of curiosity.
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