This totally up to date version targets not solely these assays instantly concerned within the discovery of GPCR-active compounds but in addition these concerned in cell-based experiments designed to check physiological responses. Whether or not coming from academia or business, or being an skilled researcher or a newcomer to the sector, the reader will discover accessible strategies and protocols that cowl the most recent developments on receptor purification, molecular biology, recombinant engineering, and analytical methods that allow the actual time monitoring of the complicated GPCR signaling cascade and identification of potential drug targets. Written for the extremely profitable Strategies in Molecular Biology sequence, chapters embrace introductions to their respective matters, lists of the mandatory supplies and reagents, step-by-step, readily reproducible laboratory protocols, and recommendations on troubleshooting and avoiding identified pitfalls.
Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Strategies and Protocols, Second Version goals to offer the instruments essential to contribute to the development of GPCR analysis and discovery and finally result in the supply of revolutionary and extra environment friendly medicine.
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